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KMID : 0921620100400030103
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Journal of Bacteriology and Virology
2010 Volume.40 No. 3 p.103 ~ p.109
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Identification of Helicobacter pylori Strain 51 Major Outer Membrane Proteins by Quadrupole Time of Flight Mass Spectrometry
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Kim Kyung-Mi
Lee Seung-Gyu
Cho Young-A
Song Yun-Gyu
Song Jae-Young
Kang Hyung-Lyun
Lee Woo-Kon
Cho Myung-Je
Rhee Kwang-Ho
Baik Seung-Chul
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Abstract
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As part of an initial inquiry into the function of the outer membrane proteins (OMPs) of Helicobacter pylori Korean strain 51, we have conducted an extensive proteome analysis via quadrupole time of flight (Q-TOF) mass spectrometry (MS). Fifty one OMPs of H. pylori were purified using sarcosine and resolved via two-dimensional electrophoresis with immobilized pH gradient strips. The most abundant proteins were observed in the alkaline pI regions (6.0~11.0) at molecular masses between 10~100 KDa. Here, 15 spots were identified, representing 9 types of genes (KHP0852, KHP0853, KHP1353, KHP1017, KHP0172, KHP0076, KHP0617, KHP1069, KHP0614) from the sarcosin-insoluble fraction of H. pylori 51. These may be employed in the characterization of the OMPs of H. pylori 51, which will help to identify new potential target proteins for vaccine development and drug therapy.
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KEYWORD
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Helicobacter pylori, Outer membrane protein, Q-TOF MS
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